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Sysmex Corporation diff quick staining reagents
Diff Quick Staining Reagents, supplied by Sysmex Corporation, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/diff-quick/diff+quick/pm42120584-163-7-10
Average 86 stars, based on 1 article reviews
diff quick staining reagents - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Incubation:

Article Title: Recombinant thrombomodulin and recombinant antithrombin attenuate pulmonary endothelial glycocalyx degradation and neutrophil extracellular trap formation in ventilator-induced lung injury in the context of endotoxemia.
Article Snippet: Cytocentrifuge slides of BALF cells were fixed in methanol and stained with Diff-Quick (Sysmex Co., Hyogo, Japan).

Article Title: Anti-transferrin receptor 1 antibody reduces angiotensin II-induced vascular remodeling.
Article Snippet: After culturing in serum-free medium for 24 h, HASMCs were incubated in the absence or presence of PPMX-T003 (1000 ng/mL) for 24 h. Then, cells were treated with 0.1 μM AngII, wounded using a yellow pipette tip in the presence or absence of PPMXT003, and cultured for 24 h. After that, the wounded cultures were stained with Diff-Quick (Sysmex, Kobe, Japan) and photographed.

Article Title: Fibroblasts activation by embryonic signal switching: A novel mechanism of placental growth factor-induced cardiac remodeling.
Article Snippet: Introduction: Cardiac remodeling is defined as cellular interstitial changes that lead dysfunction of the heart after injury.. Placental growth factor (PlGF), a member of the VEGF family, has been reported to regulate cardiac hypertrophy in hemodynamic state.. We therefore analyze the function of PlGF during cardiac remodeling using cardiac cells and fibroblasts, under Angiotensin II (AngII) stimulation.

Article Title: ASPSCR1::TFE3-mediated upregulation of insulin receptor substrate 2 (IRS-2) activates PI3K/AKT signaling and promotes malignant phenotype.
Article Snippet: The ASPSCR1::TFE3 fusion gene, resulting from chromosomal translocation, is detected in alveolar soft part sarcoma (ASPS) and a subset of renal cell carcinomas (RCC).. The ASPSCR1::TFE3 oncoprotein, functioning as an aberrant transcription factor, contributes to tumor development and progression by inappropriately upregulating target genes.. Here, we identified insulin receptor substrate 2 (IRS-2), a cytoplasmic adaptor protein, as a novel transcriptional target of ASPSCR1::TFE3.

Article Title: Allergic-Specific Immunotherapy Using Injectable In Situ Crosslinked Hyaluronic Acid Hydrogels Ameliorates Allergic Response in Murine Allergic Rhinitis Model
Article Snippet: Cells fixed on a slide, stained with Diff-quick (Sysmex Corporation, Kobe, Japan), and scanned using a digital slide scanner (Grundium Ocus ® ; Grundium, Tampere, Finland) to record the total eosinophil count.

Article Title: Particulate matter exposure induces pulmonary T H 2 responses and oxidative stress-mediated NRF2 activation in mice.
Article Snippet: Introduction: Particulate matter (PM) is a harmful air pollutant associated with respiratory and cardiovascular diseases, but its effects on adaptive immunity are poorly understood.. Objectives: This study investigates the role of NRF2 in T cells in mediating immune and pulmonary responses to long-term PM exposure, highlighting its impact on inhalation toxicity.. Methods: To establish a mouse model of lung injury induced by PM exposure, C57BL/6 mice were intranasally administered 20 μg/kg PM10 or PM2.5 daily for 16 weeks.

Article Title: Establishment and characterization of a new human gallbladder cancer cell line, OCUG-2.
Article Snippet: After incubation for 72 hours, cells that migrated to the lower compartment were stained with Diff-Quick (Sysmex Co., Kobe, Japan) and counted.

Staining:

Article Title: Recombinant thrombomodulin and recombinant antithrombin attenuate pulmonary endothelial glycocalyx degradation and neutrophil extracellular trap formation in ventilator-induced lung injury in the context of endotoxemia.
Article Snippet: Cytocentrifuge slides of BALF cells were fixed in methanol and stained with Diff-Quick (Sysmex Co., Hyogo, Japan).

Article Title: Anti-transferrin receptor 1 antibody reduces angiotensin II-induced vascular remodeling.
Article Snippet: After culturing in serum-free medium for 24 h, HASMCs were incubated in the absence or presence of PPMX-T003 (1000 ng/mL) for 24 h. Then, cells were treated with 0.1 μM AngII, wounded using a yellow pipette tip in the presence or absence of PPMXT003, and cultured for 24 h. After that, the wounded cultures were stained with Diff-Quick (Sysmex, Kobe, Japan) and photographed.

Article Title: Fibroblasts activation by embryonic signal switching: A novel mechanism of placental growth factor-induced cardiac remodeling.
Article Snippet: Introduction: Cardiac remodeling is defined as cellular interstitial changes that lead dysfunction of the heart after injury.. Placental growth factor (PlGF), a member of the VEGF family, has been reported to regulate cardiac hypertrophy in hemodynamic state.. We therefore analyze the function of PlGF during cardiac remodeling using cardiac cells and fibroblasts, under Angiotensin II (AngII) stimulation.

Article Title: ASPSCR1::TFE3-mediated upregulation of insulin receptor substrate 2 (IRS-2) activates PI3K/AKT signaling and promotes malignant phenotype.
Article Snippet: The ASPSCR1::TFE3 fusion gene, resulting from chromosomal translocation, is detected in alveolar soft part sarcoma (ASPS) and a subset of renal cell carcinomas (RCC).. The ASPSCR1::TFE3 oncoprotein, functioning as an aberrant transcription factor, contributes to tumor development and progression by inappropriately upregulating target genes.. Here, we identified insulin receptor substrate 2 (IRS-2), a cytoplasmic adaptor protein, as a novel transcriptional target of ASPSCR1::TFE3.

Article Title: Allergic-Specific Immunotherapy Using Injectable In Situ Crosslinked Hyaluronic Acid Hydrogels Ameliorates Allergic Response in Murine Allergic Rhinitis Model
Article Snippet: Cells fixed on a slide, stained with Diff-quick (Sysmex Corporation, Kobe, Japan), and scanned using a digital slide scanner (Grundium Ocus ® ; Grundium, Tampere, Finland) to record the total eosinophil count.

Article Title: Particulate matter exposure induces pulmonary T H 2 responses and oxidative stress-mediated NRF2 activation in mice.
Article Snippet: Introduction: Particulate matter (PM) is a harmful air pollutant associated with respiratory and cardiovascular diseases, but its effects on adaptive immunity are poorly understood.. Objectives: This study investigates the role of NRF2 in T cells in mediating immune and pulmonary responses to long-term PM exposure, highlighting its impact on inhalation toxicity.. Methods: To establish a mouse model of lung injury induced by PM exposure, C57BL/6 mice were intranasally administered 20 μg/kg PM10 or PM2.5 daily for 16 weeks.

Article Title: Establishment and characterization of a new human gallbladder cancer cell line, OCUG-2.
Article Snippet: After incubation for 72 hours, cells that migrated to the lower compartment were stained with Diff-Quick (Sysmex Co., Kobe, Japan) and counted.

Diff-Quik:

Article Title: Recombinant thrombomodulin and recombinant antithrombin attenuate pulmonary endothelial glycocalyx degradation and neutrophil extracellular trap formation in ventilator-induced lung injury in the context of endotoxemia.
Article Snippet: Cytocentrifuge slides of BALF cells were fixed in methanol and stained with Diff-Quick (Sysmex Co., Hyogo, Japan).

Article Title: Anti-transferrin receptor 1 antibody reduces angiotensin II-induced vascular remodeling.
Article Snippet: After culturing in serum-free medium for 24 h, HASMCs were incubated in the absence or presence of PPMX-T003 (1000 ng/mL) for 24 h. Then, cells were treated with 0.1 μM AngII, wounded using a yellow pipette tip in the presence or absence of PPMXT003, and cultured for 24 h. After that, the wounded cultures were stained with Diff-Quick (Sysmex, Kobe, Japan) and photographed.

Article Title: Fibroblasts activation by embryonic signal switching: A novel mechanism of placental growth factor-induced cardiac remodeling.
Article Snippet: Introduction: Cardiac remodeling is defined as cellular interstitial changes that lead dysfunction of the heart after injury.. Placental growth factor (PlGF), a member of the VEGF family, has been reported to regulate cardiac hypertrophy in hemodynamic state.. We therefore analyze the function of PlGF during cardiac remodeling using cardiac cells and fibroblasts, under Angiotensin II (AngII) stimulation.

Article Title: ASPSCR1::TFE3-mediated upregulation of insulin receptor substrate 2 (IRS-2) activates PI3K/AKT signaling and promotes malignant phenotype.
Article Snippet: The ASPSCR1::TFE3 fusion gene, resulting from chromosomal translocation, is detected in alveolar soft part sarcoma (ASPS) and a subset of renal cell carcinomas (RCC).. The ASPSCR1::TFE3 oncoprotein, functioning as an aberrant transcription factor, contributes to tumor development and progression by inappropriately upregulating target genes.. Here, we identified insulin receptor substrate 2 (IRS-2), a cytoplasmic adaptor protein, as a novel transcriptional target of ASPSCR1::TFE3.

Article Title: Allergic-Specific Immunotherapy Using Injectable In Situ Crosslinked Hyaluronic Acid Hydrogels Ameliorates Allergic Response in Murine Allergic Rhinitis Model
Article Snippet: Cells fixed on a slide, stained with Diff-quick (Sysmex Corporation, Kobe, Japan), and scanned using a digital slide scanner (Grundium Ocus ® ; Grundium, Tampere, Finland) to record the total eosinophil count.

Article Title: Particulate matter exposure induces pulmonary T H 2 responses and oxidative stress-mediated NRF2 activation in mice.
Article Snippet: Introduction: Particulate matter (PM) is a harmful air pollutant associated with respiratory and cardiovascular diseases, but its effects on adaptive immunity are poorly understood.. Objectives: This study investigates the role of NRF2 in T cells in mediating immune and pulmonary responses to long-term PM exposure, highlighting its impact on inhalation toxicity.. Methods: To establish a mouse model of lung injury induced by PM exposure, C57BL/6 mice were intranasally administered 20 μg/kg PM10 or PM2.5 daily for 16 weeks.

Article Title: Establishment and characterization of a new human gallbladder cancer cell line, OCUG-2.
Article Snippet: After incubation for 72 hours, cells that migrated to the lower compartment were stained with Diff-Quick (Sysmex Co., Kobe, Japan) and counted.

Transferring:

Article Title: Recombinant thrombomodulin and recombinant antithrombin attenuate pulmonary endothelial glycocalyx degradation and neutrophil extracellular trap formation in ventilator-induced lung injury in the context of endotoxemia.
Article Snippet: Cytocentrifuge slides of BALF cells were fixed in methanol and stained with Diff-Quick (Sysmex Co., Hyogo, Japan).

Article Title: Anti-transferrin receptor 1 antibody reduces angiotensin II-induced vascular remodeling.
Article Snippet: After culturing in serum-free medium for 24 h, HASMCs were incubated in the absence or presence of PPMX-T003 (1000 ng/mL) for 24 h. Then, cells were treated with 0.1 μM AngII, wounded using a yellow pipette tip in the presence or absence of PPMXT003, and cultured for 24 h. After that, the wounded cultures were stained with Diff-Quick (Sysmex, Kobe, Japan) and photographed.

Article Title: Fibroblasts activation by embryonic signal switching: A novel mechanism of placental growth factor-induced cardiac remodeling.
Article Snippet: Introduction: Cardiac remodeling is defined as cellular interstitial changes that lead dysfunction of the heart after injury.. Placental growth factor (PlGF), a member of the VEGF family, has been reported to regulate cardiac hypertrophy in hemodynamic state.. We therefore analyze the function of PlGF during cardiac remodeling using cardiac cells and fibroblasts, under Angiotensin II (AngII) stimulation.

Article Title: ASPSCR1::TFE3-mediated upregulation of insulin receptor substrate 2 (IRS-2) activates PI3K/AKT signaling and promotes malignant phenotype.
Article Snippet: The ASPSCR1::TFE3 fusion gene, resulting from chromosomal translocation, is detected in alveolar soft part sarcoma (ASPS) and a subset of renal cell carcinomas (RCC).. The ASPSCR1::TFE3 oncoprotein, functioning as an aberrant transcription factor, contributes to tumor development and progression by inappropriately upregulating target genes.. Here, we identified insulin receptor substrate 2 (IRS-2), a cytoplasmic adaptor protein, as a novel transcriptional target of ASPSCR1::TFE3.

Article Title: Allergic-Specific Immunotherapy Using Injectable In Situ Crosslinked Hyaluronic Acid Hydrogels Ameliorates Allergic Response in Murine Allergic Rhinitis Model
Article Snippet: Cells fixed on a slide, stained with Diff-quick (Sysmex Corporation, Kobe, Japan), and scanned using a digital slide scanner (Grundium Ocus ® ; Grundium, Tampere, Finland) to record the total eosinophil count.

Article Title: Particulate matter exposure induces pulmonary T H 2 responses and oxidative stress-mediated NRF2 activation in mice.
Article Snippet: Introduction: Particulate matter (PM) is a harmful air pollutant associated with respiratory and cardiovascular diseases, but its effects on adaptive immunity are poorly understood.. Objectives: This study investigates the role of NRF2 in T cells in mediating immune and pulmonary responses to long-term PM exposure, highlighting its impact on inhalation toxicity.. Methods: To establish a mouse model of lung injury induced by PM exposure, C57BL/6 mice were intranasally administered 20 μg/kg PM10 or PM2.5 daily for 16 weeks.

Article Title: Establishment and characterization of a new human gallbladder cancer cell line, OCUG-2.
Article Snippet: After incubation for 72 hours, cells that migrated to the lower compartment were stained with Diff-Quick (Sysmex Co., Kobe, Japan) and counted.

Cell Culture:

Article Title: Recombinant thrombomodulin and recombinant antithrombin attenuate pulmonary endothelial glycocalyx degradation and neutrophil extracellular trap formation in ventilator-induced lung injury in the context of endotoxemia.
Article Snippet: Cytocentrifuge slides of BALF cells were fixed in methanol and stained with Diff-Quick (Sysmex Co., Hyogo, Japan).

Article Title: Anti-transferrin receptor 1 antibody reduces angiotensin II-induced vascular remodeling.
Article Snippet: After culturing in serum-free medium for 24 h, HASMCs were incubated in the absence or presence of PPMX-T003 (1000 ng/mL) for 24 h. Then, cells were treated with 0.1 μM AngII, wounded using a yellow pipette tip in the presence or absence of PPMXT003, and cultured for 24 h. After that, the wounded cultures were stained with Diff-Quick (Sysmex, Kobe, Japan) and photographed.

Article Title: Fibroblasts activation by embryonic signal switching: A novel mechanism of placental growth factor-induced cardiac remodeling.
Article Snippet: Introduction: Cardiac remodeling is defined as cellular interstitial changes that lead dysfunction of the heart after injury.. Placental growth factor (PlGF), a member of the VEGF family, has been reported to regulate cardiac hypertrophy in hemodynamic state.. We therefore analyze the function of PlGF during cardiac remodeling using cardiac cells and fibroblasts, under Angiotensin II (AngII) stimulation.

Article Title: ASPSCR1::TFE3-mediated upregulation of insulin receptor substrate 2 (IRS-2) activates PI3K/AKT signaling and promotes malignant phenotype.
Article Snippet: The ASPSCR1::TFE3 fusion gene, resulting from chromosomal translocation, is detected in alveolar soft part sarcoma (ASPS) and a subset of renal cell carcinomas (RCC).. The ASPSCR1::TFE3 oncoprotein, functioning as an aberrant transcription factor, contributes to tumor development and progression by inappropriately upregulating target genes.. Here, we identified insulin receptor substrate 2 (IRS-2), a cytoplasmic adaptor protein, as a novel transcriptional target of ASPSCR1::TFE3.

Article Title: Allergic-Specific Immunotherapy Using Injectable In Situ Crosslinked Hyaluronic Acid Hydrogels Ameliorates Allergic Response in Murine Allergic Rhinitis Model
Article Snippet: Cells fixed on a slide, stained with Diff-quick (Sysmex Corporation, Kobe, Japan), and scanned using a digital slide scanner (Grundium Ocus ® ; Grundium, Tampere, Finland) to record the total eosinophil count.

Article Title: Particulate matter exposure induces pulmonary T H 2 responses and oxidative stress-mediated NRF2 activation in mice.
Article Snippet: Introduction: Particulate matter (PM) is a harmful air pollutant associated with respiratory and cardiovascular diseases, but its effects on adaptive immunity are poorly understood.. Objectives: This study investigates the role of NRF2 in T cells in mediating immune and pulmonary responses to long-term PM exposure, highlighting its impact on inhalation toxicity.. Methods: To establish a mouse model of lung injury induced by PM exposure, C57BL/6 mice were intranasally administered 20 μg/kg PM10 or PM2.5 daily for 16 weeks.

Article Title: Establishment and characterization of a new human gallbladder cancer cell line, OCUG-2.
Article Snippet: After incubation for 72 hours, cells that migrated to the lower compartment were stained with Diff-Quick (Sysmex Co., Kobe, Japan) and counted.



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RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with <t>Diff</t> <t>Quick</t> solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001
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Average 86 stars, based on 1 article reviews
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Image Search Results


RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Journal: Cancer Cell International

Article Title: Inhibition of RBM23 induces ferroptosis in colon cancer cells via c-Myc regulation

doi: 10.1186/s12935-026-04262-x

Figure Lengend Snippet: RBM23 knockdown inhibits colon cancer cell growth and survival (A) Protein was extracted from the normal colon cell lines CCD-18Co and colon cancer cell lines (HCT116, HT29, LOVO, LS513, DLD-1, COLO205, HCT15) and quantified in equal amounts, and then the protein expression of RBM23 was detected by western blotting. (B) The mRNA expression level of RBM23 was measured by qRT-PCR in the normal colon cell line CCD-18Co and colon cancer cell lines (DLD-1, HCT15, HT29, HCT116). GAPDH was used as an internal control for normalization. (C) Western blot analysis was performed to measure the expression levels of c-Myc in HCT15 cells treated with control siRNA or RBM23 siRNA for 72 h. (D) HCT116 cells were treated with control siRNA or RBM23 siRNA #1, #2, #3 for 72 h. Protein expression levels of various factors were then analyzed by western blotting. (E) The effect of RBM23 deficiency on HCT116, a colon cancer cell line. HCT116 was transfected with 80 nM RBM23 siRNA and control siRNA, and cell viability was examined at 0 and 72 h of treatment. Cell viability was measured by CCK8 assay and optical density was measured by microplate reader at 450 nm. Data were normalized to the control siRNA group at 0 h (set as 100%). (F) The effect of RBM23 knockdown in colon cancer cells, HCT116, on cell proliferation. HCT116 cells were transfected with RBM23 siRNA (100 nM) and incubated for one week, and colonies were stained with Diff Quick solution. The colonies were counted and quantified. (G) Western blotting was performed to detect proteins extracted from HCT116 cells fractionated into nucleus and cytoplasm. Proteins from HCT116 cells transfected with RBM23 siRNA (100 nM) for 72 h were extracted and separated by NE-PER nuclear and cytoplasmic extraction kit into proteins extracted from the nucleus and cytoplasm. The proteins were then detected using western blotting. Student’s t -test: ***P < 0.001

Article Snippet: The plates were then incubated at 37 °C, 5% CO 2 for 1 week and the colonies were fixed and stained using Diff-Quick Kit (Sysmex Corporation, Kobe, Hyogo, Japan) and the number of colonies was counted.

Techniques: Knockdown, Expressing, Western Blot, Quantitative RT-PCR, Control, Transfection, CCK-8 Assay, Incubation, Staining, Diff-Quik, Extraction